Technical assistance to assess the potential of renewable liquid and gaseous transport fuels of non-biological origin (RFNBOs) as well as recycled carbon fuels (RCFs), to establish a methodology to determine the share of renewable energy from RFNBOs as well as to develop a framework on additionality in the transport sector

This report is a summary of the work conducted in Task 1 of the technical assistance to assess the potential of renewable fuels of non-biological origin (RFNBOs) and recycled carbon fuels (RCFs) to establish a methodology to determine the share of renewable energy from RFNBOs as well as to develop a framework on additionality in the transport sector. The goal of Task 1 within the entire project was the assessment of the deployment potential of RFNBOs and RCFs over the period from 2020 to 2050 in the EU transport sector. All relevant transport sub-sectors and modalities are considered: road transport, maritime and inland shipping, aviation, and railway. Furthermore, the competition for RFNBOs and RCFs between the transport sectors and other sectors and applications of RFNBOs is considered. A central result is the potential gross final consumption of RFNBOs and RCFs that would count towards the RES target in the transport sector. In addition, the needed resources and the arising costs for this deployment as well as the impacts on greenhouse gas emissions and local environments are analyzed. Finally, barriers to the deployment and options to overcome these are outlined

Earth Observation Product Metadata Mapping to STAC

The standard for Spatio-Temporal Asset Catalogs (STAC) has matured and is widely being adopted. Capabilities, extensibility and tool support go beyond the existing OGC EOP, OpenSearch and OData standards. Implementing a catalogue with interfaces according to a standard requires good specifications and examples. This always has been an area for improvements. The OGC EOP standards have undergone a series of evolutions to become adult, onto which young STAC standard is still on the way. With a little help, it will soon overtake its master. In this paper we present three areas of improvements and suitable solutions are presented: a) overview of metadata specifications b) STAC metadata mappings for Copernicus datasets c) generic tool for metadata extractio

High-Temperature Short-Time Treatment of Human Milk for Bacterial Count Reduction

Background: Human milk (HM) for preterm infants will often be pasteurized for cytomegalovirus (CMV) inactivation and reduction of its bacterial count. High-temperature short-time (HTST) treatment compared to standard Holder pasteurization (HoP) reduces the impact of heat treatment on bioactive HM proteins while effectively inactivating CMV. No data are available for the efficacy of bacterial count reduction using HTST treatments that are available for clinical use.Objective: To test the antiviral and antibacterial efficacy of HTST treatment protocols in HM using a modified HTST treatment device compared to standard HoP.Methods: Holder pasteurized 95 mL HM samples were inoculated with Staphylococcus aureus (ATCC 6538), Enterococcus faecalis (ATCC 29212), Pseudomonas aeruginosa (ATCC 27853), Serratia marcescens (Smarc 00697), two different strains of Klebsiella pneumoniae (ATCC 700603 and Kpn 01605) or spiked with 2 × 105 50% tissue culture infective dose of CMV (AD169) and subsequently subjected to HoP (62.5°C/30 min) or HTST treatment (62°C/5 s, 62°C/15 s, 72°C/5 s, 72°C/15 s, 87°C/2 s, and 87°C/5 s). Bacterial count was determined after treated HM was cultured for 24 h. CMV infectivity was determined by the number of specific CMV immediate early antigen stained nuclei after inoculating human fibroblasts with appropriately prepared HM samples.Results: Holder pasteurized samples revealed no growth after 24 h incubation. Viable bacterial cultures were retrieved from all tested strains after HTST treatment with the default HTST protocol (62°C/5 s) that is available for clinical use. Using other time-temperature combinations, growth rates of S. aureus, E. faecalis, P. aeruginosa, K. pneumoniae, K. pneumonia, and S. marcescens were depending on treatment time, treatment temperature, bacterial genera and strain. Only after treatment temperatures above 72°C no bacterial growth was observed. CMV was inactivated by any tested time-temperature combination.Conclusions: HTST treatment inactivates CMV in 95 mL HM samples but is less effective than HoP in bacterial count reduction at a time-temperature combination of 62°C/5 s. For a reliable bacterial count reduction HTST treatment at 87°C was required in this study

mir-181A/B-1 controls thymic selection of treg cells and tunes their suppressive capacity

The interdependence of selective cues during development of regulatory T cells (Treg cells) in the thymus and their suppressive function remains incompletely understood. Here, we analyzed this interdependence by taking advantage of highly dynamic changes in expression of microRNA 181 family members miR-181a-1 and miR-181b-1 (miR-181a/b-1) during late T-cell development with very high levels of expression during thymocyte selection, followed by massive down-regulation in the periphery. Loss of miR-181a/b-1 resulted in inefficient de novo generation of Treg cells in the thymus but simultaneously permitted homeostatic expansion in the periphery in the absence of competition. Modulation of T-cell receptor (TCR) signal strength in vivo indicated that miR-181a/b-1 controlled Treg-cell formation via establishing adequate signaling thresholds. Unexpectedly, miR-181a/b-1–deficient Treg cells displayed elevated suppressive capacity in vivo, in line with elevated levels of cytotoxic T-lymphocyte–associated 4 (CTLA-4) protein, but not mRNA, in thymic and peripheral Treg cells. Therefore, we propose that intrathymic miR-181a/b-1 controls development of Treg cells and imposes a developmental legacy on their peripheral function

Effectiveness of post-fire soil erosion mitigation treatments: a systematic review and meta-analysis

Wildfires are known to be one of the main causes of soil erosion and land degradation, and their impacts on ecosystems and society are expected to increase in the future due to changes in climate and land use. It is therefore vital to mitigate the increased hydrological and erosive response after wildfires to maintain the sustainability of ecosystems and protect the values at risk downstream from the fire-affected areas. Soil erosion mitigation treatments have been widely applied after wildfires but assessment of their effectiveness has been limited to local and regional-scale studies, whose conclusions may depend heavily on site-specific conditions. To overcome this limitation, a meta-analysis approach was applied to investigations of post-wildfire soil erosion mitigation treatments published in peer-reviewed journals. A meta-analysis database was compiled that consisted of 53 and 222 pairs of treated/untreated observations on post-fire runoff and erosion, respectively, extracted from 34 publications indexed in Scopus. The overall effectiveness of mitigation treatments, expressed as the quantitative metric ‘effect size’, was determined for both the runoff and erosion observations, and further analyzed for four different types of treatments (cover-based, barriers, seeding, and chemical treatments). The erosion observations involving cover-based treatments were analyzed for differences in effectiveness between 3 different types of mulch materials (straw, wood-based, and hydromulch) as well as between different application rates of straw and wood materials. Finally, the erosion observations were also analyzed for the overall effectiveness of post-fire year, burn severity, rainfall amount and erosivity, and ground cover. The meta-analysis results show that all four types of treatments significantly reduced post-fire soil erosion, but that only the cover and barrier treatments significantly reduced post-fire runoff. From the three different cover treatments, straw and wood mulches were significantly more effective in mitigating erosion than hydromulch. In addition, the effectiveness of both straw and wood mulches depended on their application rates. Straw mulching was less effective at rates below than above 200 g m−2, while mulching with wood materials at high rates (1300 to 1750 g m−2) produced more variable outcomes than lower rates. Results also suggest that the overall effectiveness of the treatments was greatest shortly after fire, in severely burned sites, providing or promoting the development of ground cover over 70%, and that it increased with increasing rainfall erosivity. It can be concluded that, in overall terms, the application of the studied post-fire erosion mitigation treatments represented a better choice than doing nothing, especially in sites where erosion is high. However, the meta-analysis highlights under-representation of studies on this topic outside of the USA, Spain and Portugal. It was also observed that most of the studies were conducted at hillslope scale and tested mulching (namely straw, wood and hydromulch) and/or barriers, while larger scales and other treatments were scarcely addressed. Further efforts need to be made in testing, from field and modeling studies, combinations of existing and/or emerging erosion mitigation treatments to ensure that the most adequate measures are applied after fires.publishe

A922 Sequential measurement of 1 hour creatinine clearance (1-CRCL) in critically ill patients at risk of acute kidney injury (AKI)

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